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primescripttm rt master mix kit  (TaKaRa)


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    TaKaRa primescripttm rt master mix kit
    Primescripttm Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix+kit/PrimeScript+RT+Master+Mix/pmc13035274-172-7-15
    Average 99 stars, based on 26665 article reviews
    primescripttm rt master mix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Reverse Transcription:

    Article Title: Bindarit attenuates brain injury and neuroinflammation in status epilepticus by disrupting C3/C3aR signaling and modulating microglia-astrocyte interactions.
    Article Snippet: .. Total RNA of brain tissues was extracted by using Trizol reagent (R401-01, Vazyme, Nanjing, China) and reverse transcribed to cDNA using PrimeScriptTM RT Master Mix Kit (RR036A, Takara, Kusatsu, Japan). .. RT-PCR was implemented by using SYBR Green master mixes (RR820A, Takara) on Roche LightCycler480 System (Roche, Basel, Switzerland) to measure the mRNA levels of occludin, claudin-5, inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), IL-10, Arg1, transforming growth factor-β (TGF-β), C3 and C3aR.

    Article Title: Biphasic energy dependent effects of 650 nm diode laser photobiomodulation on orthodontic tooth movement and compression zone bone remodeling in vivo.
    Article Snippet: .. Messenger RNA (mRNA) was extracted with Trizol (TaKaRa, Shiga, Japan), reverse transcribed into cDNA by a PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa, Japan), and amplified by a SYBR® Premix Ex TaqTM II kit(RR820A, TaKaRa, Japan). ..

    Article Title: The E3 ubiquitin ligase RNF180 modulates the EGFR/PI3K/AKT pathway to reduce cisplatin resistance in non-small cell lung cancer
    Article Snippet: Total RNA was extracted from cells and nude mouse tissues via TRIzol reagent (Takara, Japan). .. The extracted RNA was reverse-transcribed into cDNA with the TaKaRa PrimeScriptTM RT Master Mix Kit (Takara, Cat. No. RR036A) under the following reaction conditions: 37°C for 15 minutes, 85°C for 5 seconds, and maintained at 4°C. ..

    Article Title: The E3 ubiquitin ligase RNF180 modulates the EGFR/PI3K/AKT pathway to reduce cisplatin resistance in non-small cell lung cancer.
    Article Snippet: Real-time quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from cells and nude mouse tissues via TRIzol reagent (Takara, Japan). .. The extracted RNA was reverse-transcribed into cDNA with the TaKaRa PrimeScriptTM RT Master Mix Kit (Takara, Cat. No. RR036A) under the following reaction conditions: 37°C for 15 minutes, 85°C for 5 seconds, and maintained at 4°C. ..

    other:

    Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression
    Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific).

    Spectrophotometry:

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1 , COX-2 , and PGE2 using the SYBR ® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway.
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1, COX-2, and PGE2 using the SYBR® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Synthesized:

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1 , COX-2 , and PGE2 using the SYBR ® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway.
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1, COX-2, and PGE2 using the SYBR® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1 , COX-2 , and PGE2 using the SYBR ® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway.
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1, COX-2, and PGE2 using the SYBR® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1 , COX-2 , and PGE2 using the SYBR ® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway.
    Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa Bio, Shiga, Japan). qRT-PCR was subsequently performed for ELAVL1, COX-2, and PGE2 using the SYBR® Premix Ex TaqTM II (RR820A, TaKaRa Bio, Japan) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Amplification:

    Article Title: Biphasic energy dependent effects of 650 nm diode laser photobiomodulation on orthodontic tooth movement and compression zone bone remodeling in vivo.
    Article Snippet: .. Messenger RNA (mRNA) was extracted with Trizol (TaKaRa, Shiga, Japan), reverse transcribed into cDNA by a PrimeScriptTM RT Master Mix kit (RR036A, TaKaRa, Japan), and amplified by a SYBR® Premix Ex TaqTM II kit(RR820A, TaKaRa, Japan). ..

    cDNA Synthesis:

    Article Title: Food-grade TiO 2 /TDCPP Co-exposure Disrupts ACOD1/Itaconate Axis and Is Associated with TET2-NF-κB Inflammation in Microglia Exacerbating Neurotoxicity.
    Article Snippet: Food-grade titanium dioxide (TiO2) and tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) are environmental pollutants with high exposure among children, which pose potential risks to neurodevelopment.. However, the neurotoxicity of their co-exposure remains poorly understood.. In this study, we employed C8-D1A astrocytes, BV2 microglia, and Neuro-2a neuroblastoma cells to investigate the neurotoxicity of food-grade TiO2 and TDCPP co-exposure.



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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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    Image Search Results


    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Journal: Frontiers in Neurology

    Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

    doi: 10.3389/fneur.2026.1793054

    Figure Lengend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Article Snippet: Total RNA was extracted from patients and their parents using the Qiagen RNA Preparation Kit, and complementary DNA (cDNA) was synthesized by reverse transcription using the PrimeScriptTM Strand cDNA Synthesis Kit/RT Master Mix (TAKARA).

    Techniques: Expressing, Variant Assay, Amplification