primescripttm rt master mix kit (TaKaRa)
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Primescripttm Rt Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 26665 article reviews
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Reverse Transcription:Article Title: Bindarit attenuates brain injury and neuroinflammation in status epilepticus by disrupting C3/C3aR signaling and modulating microglia-astrocyte interactions. Article Snippet: .. Total RNA of brain tissues was extracted by using Trizol reagent (R401-01, Vazyme, Nanjing, China) and reverse transcribed to cDNA using Article Title: Biphasic energy dependent effects of 650 nm diode laser photobiomodulation on orthodontic tooth movement and compression zone bone remodeling in vivo. Article Snippet: .. Messenger RNA (mRNA) was extracted with Trizol (TaKaRa, Shiga, Japan), reverse transcribed into cDNA by a Article Title: The E3 ubiquitin ligase RNF180 modulates the EGFR/PI3K/AKT pathway to reduce cisplatin resistance in non-small cell lung cancer Article Snippet: Total RNA was extracted from cells and nude mouse tissues via TRIzol reagent (Takara, Japan). .. The extracted RNA was reverse-transcribed into cDNA with the TaKaRa Article Title: The E3 ubiquitin ligase RNF180 modulates the EGFR/PI3K/AKT pathway to reduce cisplatin resistance in non-small cell lung cancer. Article Snippet: Real-time quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted from cells and nude mouse tissues via TRIzol reagent (Takara, Japan). .. The extracted RNA was reverse-transcribed into cDNA with the TaKaRa other:Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression Article Snippet: The samples were treated with DNase I, using the RNase free DNase kit (Qiagen1023460) and RNA concentration determined with a Nanodrop 2000 (Thermo Fisher Scientific). Spectrophotometry:Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway. Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Synthesized:Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway. Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Quantitative RT-PCR:Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway. Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Real-time Polymerase Chain Reaction:Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek’s Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Article Title: ELAVL1 Promotes Proliferation and Inhibits Apoptosis of the Marek's Disease Virus (MDV)-Transformed Cell Line MSB1 via the COX-2/PGE2 Pathway. Article Snippet: .. Briefly, total RNA was extracted from MSB1 cells in each group at 48 h post-transfection using TRIzol (15596018CN, Invitrogen, Carlsbad, CA, USA) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). cDNA was synthesized from 1 μg of total RNA using the Amplification:Article Title: Biphasic energy dependent effects of 650 nm diode laser photobiomodulation on orthodontic tooth movement and compression zone bone remodeling in vivo. Article Snippet: .. Messenger RNA (mRNA) was extracted with Trizol (TaKaRa, Shiga, Japan), reverse transcribed into cDNA by a cDNA Synthesis:Article Title: Food-grade TiO 2 /TDCPP Co-exposure Disrupts ACOD1/Itaconate Axis and Is Associated with TET2-NF-κB Inflammation in Microglia Exacerbating Neurotoxicity. Article Snippet: Food-grade titanium dioxide (TiO2) and tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) are environmental pollutants with high exposure among children, which pose potential risks to neurodevelopment.. However, the neurotoxicity of their co-exposure remains poorly understood.. In this study, we employed C8-D1A astrocytes, BV2 microglia, and Neuro-2a neuroblastoma cells to investigate the neurotoxicity of food-grade TiO2 and TDCPP co-exposure. |
